Artemis is the first company to have developed a robust methodology for the controlled induction of shRNA based gene knock down (6) in adult mice. This inducible system permits the down-regulation of a selected target gene to be turned on and off thereby more closely modeling the dosing of a pharmacologic inhibitor. The generation time for such a mouse model at Artemis is only four months.
Using a reporter system, we have demonstrated that in our system constitutive RNAi knock down of over 80% can be achieved in almost all tissues of the body. Of what protein?
This knock down has been shown to be stable over at least 25 weeks and is inheritable into the next generation. At 80% KO strength?
Development of a system to efficiently produce RNAi knock down mice within 4 months. Why does it take less time than making other genetically altered mice?
We have shown that RNAi-based constitutive knock down of endogenous genes can reproduce the phenotype of the corresponding conventional gene KO. Such as???